Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen
Author
Summary, in English
Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.
Publishing year
1999
Language
English
Pages
1-12
Publication/Series
Tumor Biology
Volume
20
Issue
Suppl. 1
Document type
Journal article
Publisher
Springer
Topic
- Cancer and Oncology
Keywords
- PSA
- Prostate
- Antibody
- Workshop
- ISOBM
Status
Published
Research group
- Clinical Chemistry, Malmö
- Celiac Disease and Diabetes Unit
ISBN/ISSN/Other
- ISSN: 1423-0380