The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

A family of complex tandem DNA repeats in the telomeres of Chironomus pallidivittatus

På svenska

Author

  • Y-J Zhang
  • I Kamnert
  • C Lopéz
  • Marita Cohn
  • Jan-Erik Edström

Summary, in English

A family of 340-bp tandem telomere-associated DNA repeats is present in 50- to 200-kb blocks in seven of the eight paired chromosome ends in Chironomus pallidivittatus. It consists of four main subfamilies, differing from each other by small clusters of mutations. This differentiation may reflect different functional roles for the repeats. Here we find that one subfamily, D3, is consistently localized most peripherally and extends close to the ends of the chromosomes, as shown by its sensitivity to the exonuclease Bal 31. The amounts of D3 are highly variable between individuals. The repeat characteristic for D3 forms a segment with pronounced dyad symmetry, which in single-strand form would give rise to a hairpin. Evidence from an interspecies comparison suggests that a similar structure is the result of selective forces. Another subfamily, M1, is present more proximally in a subgroup of telomeres characterized by a special kind of repeat variability. Thus, a complex block with three kinds of subfamilies may occupy different M1 telomeres depending on the stock of animals. We conclude that subfamilies are differentially distributed between and within telomeres and are likely to serve different functions.

Publishing year

1994

Language

English

Pages

8028-8036

Publication/Series

Molecular and Cellular Biology

Volume

14

Issue

12

Document type

Journal article

Publisher

American Society for Microbiology

Topic

  • Genetics

Status

Published

Research group

  • Molecular Genetics and Genetics

ISBN/ISSN/Other

  • ISSN: 0270-7306