Serum cystatin C, determined by a rapid, automated particle-enhanced turbidimetric method, is a better marker than serum creatinine for glomerular filtration rate

Summary, in English

We describe a fully automated particle-enhanced turbidimetric assay for cystatin C in undiluted serum and EDTA-plasma. The throughput is 90 samples per hour and urgent samples can be analyzed in 7 min. The assay range (0.4-14.1 mg/L) covers the concentration range in health and disease. The within- and between-run imprecision is 0.9% and 2.2%, respectively. Analytical recovery of additions of recombinant cystatin C averaged 98%. Rheumatoid factors (< or = 323,000 IU/L), bilirubin (< or = 150 mumol/L), hemoglobin (< or = 1.2 g/L), and triglycerides (< or = 8.5 mmol/L) do not interfere in the assay. In view of the superior (by ROC analysis) diagnostic accuracy of serum concentrations of cystatin C for reduced glomerular filtration rate (GFR) in comparison with creatinine, cystatin C seems an attractive alternative to creatinine for estimation of GFR.

• Author: Jan Kyhse-Andersen; Camilla Schmidt; Gunnar Nordin; Britta Andersson; Peter Nilsson-Ehle; Veronica Lindström; Anders Grubb
• Department/s: Division of Clinical Chemistry and Pharmacology
• Publishing year: 1994
• Language: English
• Pages: 1921-1926
• Publication/Series: Clinical Chemistry
• Volume: 40
• Issue: 10
• Links: http://www.clinchem.org/cgi/reprint/40/10/1921
• Document type: Journal article
• Publisher: American Association for Clinical Chemistry
• Topic: Biochemistry and Molecular Biology
• Status: Published
• ISBN/ISSN/Other: ISSN: 0009-9147