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New antimicrobial peptide active against Gram-positive pathogens

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Author

Summary, in English

Background & objectives: Human and animal cystatins have been shown to inhibit the replication of certain viruses and bacteria, though it is not directly demonstrated that the effects are due to protease inhibitory capacity of the cystatins. We report antibacterial properties of a novel antimicrobial peptidyl derivative, (2S)-2-(N-alpha-benzyloxycarbonyl-arginyl-leucylamido)-1-(E)-cinnamoyla mido-3-methylbutane, structurally based upon the aminoterminal segment of the inhibitory centre of the human cysteine protease inhibitor, cystatin C. Methods: Clinical isolates of group A, B, C and G streptococci were collected. The antibacterial activity of Cystapep 1 derivative was tested by agar well diffusion method. Results: Cystapep 1, displayed antibacterial activity against several clinically important Gram-positive bacteria. It displayed minimal inhibitory and bactericidal concentrations of about 16 mug/ml for both Staphylococcus aureus and Streptococcus pyogenes. In radial agar diffusion assays, groups A, B, C and G streptococci as well as staphylococci were generally susceptible to the action of Cystapep 1, whereas pneumococci and enterococci were less susceptible. No activity against Gram-negative bacteria was observed. Interpretation & conclusion: Cystapep 1 also showed high activity against methicillin-resistant Staph. aureus (MRSA) and multi-antibiotic resistant coagulase negative staphylococci (CNS), suggesting its mechanism of action to be different from most currently used antibiotics.

Publishing year

2004

Language

English

Pages

74-76

Publication/Series

Indian Journal of Medical Research

Volume

119

Issue

Suppl.

Document type

Journal article

Publisher

Council Of Medical Research

Topic

  • Microbiology in the medical area
  • Pharmacology and Toxicology
  • Medicinal Chemistry

Keywords

  • Staphylococcus aureus
  • antimicrobial peptide
  • cystatin C
  • Streptococcus
  • pyogenes

Status

Published

ISBN/ISSN/Other

  • ISSN: 0971-5916