Differential gene expression in adipose tissue from obese human subjects during weight loss and weight maintenance.
Author
Summary, in English
BACKGROUND:
Differential gene expression in adipose tissue during diet-induced weight loss followed by a weight stability period is poorly characterized. Markers of these processes may provide a deeper understanding of underlying mechanisms.
OBJECTIVE:
We aimed to identify differentially expressed genes in human adipose tissue during weight loss and weight maintenance after weight loss.
DESIGN:
RNA from subcutaneous abdominal adipose tissue from 9 obese subjects was analyzed by using a complementary DNA microarray at baseline after weight loss on a low-calorie diet and after weight maintenance.
RESULTS:
Subjects lost 18.8 ± 1.8% of weight and maintained this loss during weight maintenance (1.1 ± 2.1%; range: -9.3 to 10.6%). Most differentially expressed genes exhibited a reciprocal regulation and returned to baseline after weight loss (2163 genes) and weight maintenance (3175 genes). CETP and ABCG1, both of which participate in the HDL-mediated reverse cholesterol transport (RCT), were among the most upregulated of the 750 genes that were differentially expressed after both processes. Several genes involved in inflammation were downregulated. The use of real-time polymerase chain reaction confirmed or partially confirmed the previously implicated genes TNMD and MMP9 (both downregulated), PNPLA3 (upregulated), and CIDEA and SCD (both reciprocally regulated).
CONCLUSIONS:
The beneficial effects of weight loss should be investigated after long-term weight maintenance. The processes of weight loss and weight maintenance should be viewed as biologically distinct. CETP and ABCG1 may be important mediators of these effects through HDL-mediated RCT.
Differential gene expression in adipose tissue during diet-induced weight loss followed by a weight stability period is poorly characterized. Markers of these processes may provide a deeper understanding of underlying mechanisms.
OBJECTIVE:
We aimed to identify differentially expressed genes in human adipose tissue during weight loss and weight maintenance after weight loss.
DESIGN:
RNA from subcutaneous abdominal adipose tissue from 9 obese subjects was analyzed by using a complementary DNA microarray at baseline after weight loss on a low-calorie diet and after weight maintenance.
RESULTS:
Subjects lost 18.8 ± 1.8% of weight and maintained this loss during weight maintenance (1.1 ± 2.1%; range: -9.3 to 10.6%). Most differentially expressed genes exhibited a reciprocal regulation and returned to baseline after weight loss (2163 genes) and weight maintenance (3175 genes). CETP and ABCG1, both of which participate in the HDL-mediated reverse cholesterol transport (RCT), were among the most upregulated of the 750 genes that were differentially expressed after both processes. Several genes involved in inflammation were downregulated. The use of real-time polymerase chain reaction confirmed or partially confirmed the previously implicated genes TNMD and MMP9 (both downregulated), PNPLA3 (upregulated), and CIDEA and SCD (both reciprocally regulated).
CONCLUSIONS:
The beneficial effects of weight loss should be investigated after long-term weight maintenance. The processes of weight loss and weight maintenance should be viewed as biologically distinct. CETP and ABCG1 may be important mediators of these effects through HDL-mediated RCT.
Department/s
- Diabetes - Clinical Obesity
- Translational Muscle Research
- EXODIAB: Excellence of Diabetes Research in Sweden
Publishing year
2012
Language
English
Pages
196-207
Publication/Series
The American journal of clinical nutrition
Volume
96
Issue
1
Links
Document type
Journal article
Publisher
Oxford University Press
Topic
- Nutrition and Dietetics
Status
Published
Research group
- Diabetes - Clinical Obesity
- Genomics, Diabetes and Endocrinology
ISBN/ISSN/Other
- ISSN: 1938-3207