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False positive results in class-specific rheumatoid factor (RF) assays due to interaction between RF and Fc fragments of anti-immunoglobulin indicator reagents

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Author

Summary, in English

A recently developed, simple immunological method, diffusion-in-gel ELISA, has been adapted for class-specific determination of rheumatoid factor (RF). Evidence was obtained of an analytical error due to interaction between RF and antigenic determinants on the Fc part of the indicator immunoglobulin (Ig) molecules used. This was eliminated by replacing the usual indicator reagent, complete Ig molecules, by conjugated Fab or F(ab')2 fragments. The findings imply that unless the RF-Fc interaction mentioned is avoided in the assaying technique, false positive results may be obtained for, e.g. IgG RF and IgA RF in sera containing IgM RF.

Publishing year

1983

Language

English

Pages

171-182

Publication/Series

Journal of Immunological Methods

Volume

58

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Immunology in the medical area

Keywords

  • rheumatoid factor
  • immunoassay
  • immunoglobulin class

Status

Published

ISBN/ISSN/Other

  • ISSN: 1872-7905