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A stable, radioactive substrate emulsion for assay of lipoprotein lipase

På svenska

Author

Summary, in English

A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and suitable for routine use.

Publishing year

1976

Language

English

Pages

536-541

Publication/Series

Journal of Lipid Research

Volume

17

Issue

5

Document type

Journal article

Publisher

American Society for Biochemistry and Molecular Biology

Topic

  • Medicinal Chemistry
  • Pharmacology and Toxicology

Keywords

  • adipose lipoprotein lipase tri[3H]oleoylglycerol
  • triglyceride
  • postheparin serum

Status

Published

ISBN/ISSN/Other

  • ISSN: 1539-7262