S-100beta stimulates neurite outgrowth in the rat sciatic nerve grafted with acellular muscle transplants
Author
Summary, in English
S-100beta promotes neurite extension in vitro and motoneuron survival in the chicken embryo. We demonstrate here that local administration of S-100beta stimulates the sciatic nerve regeneration into acellular muscle grafts. Normally there is a 8-10 day delay in the regeneration of axons into such grafts. Local administration of S-100beta (0.5-1.0 microg/h) significantly stimulated regeneration into the grafts. In S-100beta treated grafts, the regeneration distance was increased with a factor of about 2.3 times as compared to vehicle treated grafts. The distance of regeneration was monitored with pinch test which detects sensory axons. Regenerating axons were growing outside the necrotic muscle cells as revealed with immunohistochemistry for the neurofilament light weight polypeptide. S-100beta was demonstrated immunocytochemically in motor neurons of the rat lumbar spinal cord and in large and medium sized neurons of the dorsal root ganglia. The results suggest that S-100beta is a physiological growth factor for peripheral nerve axons.
Department/s
- Department of Clinical Sciences, Malmö
- Neural Interfaces
Publishing year
1997
Language
English
Pages
196-201
Publication/Series
Brain Research
Volume
753
Issue
2
Document type
Journal article
Publisher
Elsevier
Topic
- Neurosciences
Keywords
- Axotomy
- Nerve regeneration
- Neurotrophic effect
- Sensory test
Status
Published
Research group
- Neural Interfaces
ISBN/ISSN/Other
- ISSN: 1872-6240