The bacterial flora of fresh and chill-stored pork: analysis by cloning and sequencing of 16S rRNA genes.
Author
Summary, in English
The composition of the initial bacterial flora of pork and the development of the flora after storage at +4 °C for 4 days were analysed by amplification, cloning and sequencing of 16S rDNA. A total of 122 clones were obtained, with lengths of ≥400 nucleotides and ≥95% similarity to database sequences. Nineteen clones were similar to sequences in database not assigned to any genera. Fourteen different genera were represented in clones from fresh meat, with 36.5% of the clones most resembling Acinetobacter and 17.3% resembling Staphylococcus and Macrococcus. After storage, the clones were composed of six different genera, with 44.3% resembling Pseudomonas, 17.1% resembling Aeromonas and only 14.3% resembling Acinetobacter. This study shows that the overall pattern of the initial and chill-stored pork flora, as shown by a molecular approach, was in agreement with results obtained in previous studies using traditional cultivation methods.
Department/s
- Department of Food Technology, Engineering and Nutrition
Publishing year
2003
Language
English
Pages
245-252
Publication/Series
International Journal of Food Microbiology
Volume
83
Issue
3
Document type
Journal article
Publisher
Elsevier
Topic
- Food Engineering
Keywords
- Pork
- Eperythrozoon
- Diversity
- 16S rDNA cloning
Status
Published
ISBN/ISSN/Other
- ISSN: 0168-1605