Carbohydrate-deficient transferrin measured by high-performance liquid chromatography and CDTect immunoassay

Summary, in English

Carbohydrate-deficient transferrin (CDT) was measured in three populations using anion-exchange chromatography followed by radioimmunoassay (CDTectTM) and a new high-performance liquid chromatography (HPLC) method. The correlation between the methods in 50 consecutive clinical samples was good (r = 0.87). However, in a set of 49 samples with CDT concentrations close to the reference value of the methods, the correlation was low (r = 0.51). In addition, among controls, no correlation between the methods was found (r = 0.10). Abnormal isoforms of transferrin were noted in 5% of the clinical samples. HPLC determination of CDT offers the advantage of identifying these isoforms of transferrin and thus reducing analytical pitfalls associated with the CDTectTM method.

• Author: Per Simonsson; S Lindberg; Christer Alling
• Department/s: Clinical Chemistry, Malmö; Division of Clinical Chemistry and Pharmacology
• Publishing year: 1996
• Language: English
• Pages: 397-402
• Publication/Series: Alcohol and Alcoholism
• Volume: 31
• Issue: 4
• Links: http://alcalc.oxfordjournals.org/cgi/reprint/31/4/397.pdf
• Document type: Journal article
• Publisher: Oxford University Press
• Topic: Substance Abuse
• Status: Published
• Research group: Clinical Chemistry, Malmö
• ISBN/ISSN/Other: ISSN: 1464-3502