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beta-Microseminoprotein binds CRISP-3 in human seminal plasma

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Author

Summary, in English

P-Microseminoprotein (MSP) and cysteine-rich secretory protein 3 (CRISP-3) are abundant constituents of human seminal plasma. Immunoprecipitation and gel filtration of seminal plasma proteins combined with examination of the proteins in their pure form showed that MSP and CRISP-3 form stable, non-covalent complexes. CRISP-3 binds MSP with very high affinity, as evidenced by surface plasmon resonance. Due to far higher abundance of MSP in prostatic fluid, it manifests large overcapacity for CRISP-3 binding. Structural similarity with an MSP-binding protein from blood plasma suggests that CRISP-3 binds MSP through its ami-terminal SCP-domain.

Publishing year

2005

Language

English

Pages

555-561

Publication/Series

Biochemical and Biophysical Research Communications

Volume

333

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • mass
  • spectrometry
  • surface plasmon resonance
  • SCP-domain
  • protein complex
  • prostate cancer
  • seminal plasma
  • PSP94
  • cysteine-rich secretory protein
  • beta-microseminoprotein

Status

Published

Research group

  • Clinical Chemistry, Malmö
  • Urological cancer, Malmö

ISBN/ISSN/Other

  • ISSN: 1090-2104