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Mapping and cloning of FAD2 gene to develop allele-specific PCR for oleic acid in spring turnip rape (Brassica rapa ssp. oleifera)

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Author

Summary, in English

The previously identified QTL for oleic acid content observed in an Fz population from the Brassica mpn ssp. oleifera cross Jo4002 x Jo4072 (a high-oleic-acid individual) was mapped more precisely by adding markers to the linkage group which harbours the locus. In addition, the fad2 gene, which is known to encode the 18:1 desaturase in Arabidopsis, was mapped in Brassica, too. The results are consistent with the QTL corresponding to the Arabiciopsis fad2 gene. Comparison of the wild-type and high-oleic-acid allele of the locus revealed only one difference in their nucleic acid sequences leading to an amino acid change. This substitution of leucine by proline most likely affects the fold of the protein and thereby activity of the enzyme. Using this base difference, an allele-specific PCR was designed. The allele-specific markers will be very effective in selection for plants with high-oleic-acid content derived from Jo4072 because they are located exactly at the locus and can differentiate between homo- and heterozygotes.

Publishing year

1998

Language

English

Pages

543-550

Publication/Series

Molecular Breeding

Volume

4

Issue

6

Document type

Journal article

Publisher

Springer

Topic

  • Medical Genetics

Keywords

  • allele-specific PCR
  • Brassica rapa
  • fad2
  • marker-assisted selection
  • oleic acid

Status

Published

ISBN/ISSN/Other

  • ISSN: 1380-3743