Lysophosphatidic Acid Binds to and Activates GPR92, a G Protein-Coupled Receptor Highly Expressed in Gastrointestinal Lymphocytes

Summary, in English

Here, the ligand binding, activation, and tissue distribution of the orphan G protein-coupled receptor (GPCR) GPR92 were studied. GPR92 binds and is activated by compounds based on the lysophosphatidic acid (LPA) backbone. The binding of LPA to GPR92 was of high affinity (K(D) = 6.4 +/- 0.9 nM) and led to an increase in both phosphoinositide hydrolysis and cAMP production. GPR92 is atypical in that it has a low sequence homology with the classic LPA(1-3) receptors (21-22%). Expression of GPR92 is mainly found in heart, placenta, spleen, brain, lung, and gut. Notably, GPR92 is highly expressed in the lymphocyte compartment of the gastrointestinal tract. It is the most abundant GPCR activated by LPA found in the small intestinal intraepithelial CD8+ cytotoxic T cells.

• Author: Knut Kotarsky; Ake Boketoft; Jesper Bristulf; Niclas Nilsson; Ake Norberg; Stefan Hansson; Rannar Sillard; Christer Owman; Fredrik Leeb-Lundberg; Björn Olde
• Department/s: Mucosal Immunology; Department of Experimental Medical Science; Obstetrics and Gynaecology (Lund); Drug Target Discovery
• Publishing year: 2006
• Language: English
• Pages: 619-628
• Publication/Series: Journal of Pharmacology and Experimental Therapeutics
• Volume: 318
• Issue: 2
• Links: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16651401&dopt=Abstract; http://dx.doi.org/10.1124/jpet.105.098848
• Document type: Journal article
• Publisher: American Society for Pharmacology and Experimental Therapeutics
• Topic: Immunology in the medical area
• Status: Published
• Research group: Mucosal Immunology; Drug Target Discovery
• ISBN/ISSN/Other: ISSN: 1521-0103