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LPS induces GROalpha chemokine production via NF-kappaB in oral fibroblasts.

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Author

Summary, in English

Objective and design Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GRO alpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GRO alpha by oral fibroblasts have not previously been presented. Materials and methods GRO alpha mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA. Results We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GRO alpha in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GRO alpha and GRO alpha protein. The steroid hormone estrogen had no effect on LPS-induced GRO alpha mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GRO alpha production, and the NF-kappa B blocker MG 132 fully prevented LPS-induced GRO alpha. Conclusions Oral fibroblasts respond to LPS stimulation by increasing GRO alpha production via the transcription factor NF-kappa B, suggesting that this mechanism may be involved in development of periodontal inflammation.

Publishing year

2009

Language

English

Pages

791-796

Publication/Series

Inflammation Research

Volume

58

Issue

11

Document type

Journal article

Publisher

Birkhäuser Verlag

Topic

  • Rheumatology and Autoimmunity

Keywords

  • Estrogen
  • Chemokines
  • Dexamethasone
  • GRO alpha
  • PDL cells

Status

Published

Research group

  • Vascular Physiology

ISBN/ISSN/Other

  • ISSN: 1420-908X