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Multilayered Magnetic Nanoparticles as a Support in Solid-Phase Peptide Synthesis

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Author

Summary, in English

The synthesis of multilayered magnetic nanoparticles (MNPs) for use as a support in solid-phase peptide synthesis (SPPS) is described. Silanization of magnetite (Fe3O4) nanoparticles with 3-(trimethoxysilyl)propyl methacrylate introduced polymerizable groups on the surface. Polymerization with allylamine, trimethylolpropane trimethacrylate, and trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate provided a polymeric coating and amino groups to serve as starting points for the synthesis. After coupling of an internal reference amino acid and a cleavable linker, the coated MNPs were applied as the solid phase during synthesis of Leu-enkephalinamide and acyl carrier protein (65-74) by Fmoc chemistry. A "high-load" version of the MNP support (0.32 mmol/g) was prepared by four consecutive cycles of Fmoc-Lys(Fmoc)-OH coupling and Fmoc deprotection. Successful synthesis of Leu-enkephalin was demonstrated on the "high-load" MNPs. Chemical stability studies proved the particles to be stable under SPPS conditions and magnetization measurements showed that the magnetic properties of the particles were maintained throughout derivatizations and SPPS. The MNPs were further characterized by high-resolution transmission electron microscopy, inductively coupled plasma atomic emission spectrometry, elemental analysis, and nitrogen gas adsorption measurements.

Publishing year

2009

Language

English

Pages

287-292

Publication/Series

International Journal of Peptide Research and Therapeutics

Volume

15

Issue

4

Document type

Journal article

Publisher

Springer

Topic

  • Pharmacology and Toxicology

Keywords

  • Support
  • Solid-phase peptide synthesis
  • Polymer
  • Multilayer structure
  • Magnetite
  • Magnetic materials
  • Nanocomposites

Status

Published

Research group

  • Nanomedicine and Biomaterials

ISBN/ISSN/Other

  • ISSN: 1573-3904