NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea

Summary, in English

The structure of calerythrin, a prokaryotic 20 kDa calcium-binding protein has been determined by solution NMR spectroscopy. Distance, dihedral angle, J coupling, secondary chemical shift, residual dipolar coupling and radius of gyration restraints reveal four EF-hand motifs arranged in a compact globular structure. A tight turn in the middle of the amino acid sequence brings the two halves, each comprising a pair of EF-hands, close together. The structural similarity between calerythrin and the eukaryotic sarcoplasmic calcium-binding proteins is notable.

• Author: H Tossavainen; P Permi; A Annila; I Kilpelainen; Torbjörn Drakenberg
• Department/s: Biophysical Chemistry
• Publishing year: 2003
• Language: English
• Pages: 2505-2512
• Publication/Series: European Journal of Biochemistry
• Volume: 270
• Issue: 11
• Links: http://www.ejbiochem.org/cgi/content/abstract/270/11/2505; http://dx.doi.org/10.1046/j.1432-1033.2003.03623.x
• Document type: Journal article
• Publisher: Wiley-Blackwell
• Topic: Physical Chemistry
• Keywords: calcium-binding; calerythrin; NMR; structure
• Status: Published
• ISBN/ISSN/Other: ISSN: 0014-2956