Quantification of Human Kallikrein-2 in Clinical Samples by Selected Reaction Monitoring

Summary, in English

Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL).

• Author: Ákos Végvári; Karin Sjödin; Melinda Rezeli; György Marko-Varga
• Department/s: Department of Biomedical Engineering
• Publishing year: 2013
• Language: English
• Pages: 4612-4616
• Publication/Series: Journal of Proteome Research
• Volume: 12
• Issue: 10
• Links: http://dx.doi.org/10.1021/pr400423k
• Document type: Journal article
• Publisher: The American Chemical Society (ACS)
• Topic: Medical Engineering
• Keywords: Chromosome 19; Human kallikrein-2; Selected reaction monitoring; Mass spectrometry
• Status: Published
• ISBN/ISSN/Other: ISSN: 1535-3893