In-Source Decay Causes Artifacts in SELDI-TOF MS Spectra.

Summary, in English

SELDI-TOF MS is a mass spectrometric technique which has been extensively used for biomarker discovery. In this study, we show that in-source decay is an important source for the generation of additional spectral peaks with this technique, both for pure proteins and proteins in serum samples. Thus, SELDI-TOF MS could be used to gain sequence information from proteins, but the results also question the uncritical use of SELDI-TOF MS as a general method for the detection of biomarkers.

• Author: Lars Ekblad; Bo Baldetorp; Mårten Fernö; Håkan Olsson; Charlotte Welinder
• Department/s: Tumor microenvironment; Breastcancer-genetics
• Publishing year: 2007
• Language: English
• Pages: 1609-1614
• Publication/Series: Journal of Proteome Research
• Volume: 6
• Issue: 4
• Links: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17373843&dopt=Abstract; http://dx.doi.org/10.1021/pr060633y
• Document type: Journal article
• Publisher: The American Chemical Society (ACS)
• Topic: Cancer and Oncology
• Status: Published
• ISBN/ISSN/Other: ISSN: 1535-3893