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Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor

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Author

Summary, in English

In this paper we demonstrate the presence of two novel in vivo autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR): Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore demonstrate that the adapter protein Grb2 is a specific binding partner for both phosphorylated Tyr-703 and phosphorylated Tyr-936, whereas the adapter protein Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7. Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the Ras/mitogen-activated protein kinase pathway.

Publishing year

1999

Language

English

Pages

211-216

Publication/Series

Biochemical Journal

Volume

341

Issue

1

Document type

Journal article

Publisher

Portland Press

Topic

  • Medicinal Chemistry

Keywords

  • Signal Transducing Cells
  • *Adaptor Proteins
  • Cultured Endothelium
  • Vascular/cytology GRB2 Adaptor Protein GRB7 Adaptor Protein Humans Phosphorylation Protein Binding Proteins/*metabolism Proto-Oncogene Proteins c-kit/genetics/*metabolism Receptor Protein-Tyrosine Kinases/genetics/metabolism Recombinant Fusion Proteins/metabolism Stem Cell Factor *Tyrosine src Homology Domains

Status

Published

ISBN/ISSN/Other

  • ISSN: 0264-6021