Tomosyn-1 is involved in a post-docking event required for pancreatic beta-cell exocytosis
Author
Summary, in English
Although the assembly of a ternary complex between the SNARE proteins syntaxin-1, SNAP25 and VAMP2 is known to be crucial for insulin exocytosis, the mechanisms controlling this key event are poorly understood. We found that pancreatic beta-cells express different isoforms of tomosyn-1, a syntaxin-1-binding protein possessing a SNARE-like motif. Using atomic force microscopy we show that the SNARE-like domain of tomosyn-1 can form a complex with syntaxin-1 and SNAP25 but displays binding forces that are weaker than those observed for VAMP2 (237 +/- 13 versus 279 +/- 3 pN). In pancreatic beta-cells tomosyn-1 was found to be concentrated in cellular compartments enriched in insulin-containing secretory granules. Silencing of tomosyn-1 in the rat beta-cell line INS-1E by RNA interference did not affect the number of secretory granules docked at the plasma membrane but led to a reduction in stimulus-induced exocytosis. Replacement of endogenous tomosyn-1 with mouse tomosyn-1, which differs in the nucleotide sequence from its rat homologue and escapes silencing, restored a normal secretory rate. Taken together, our data suggest that tomosyn-1 is involved in a post-docking event that prepares secretory granules for fusion and is necessary to sustain exocytosis of pancreatic beta-cells in response to insulin secretagogues.
Department/s
Publishing year
2006
Language
English
Pages
2912-2920
Publication/Series
Journal of Cell Science
Volume
119
Issue
14
Document type
Journal article
Publisher
The Company of Biologists Ltd
Topic
- Endocrinology and Diabetes
Keywords
- exocytosis
- SNARE
- insulin
- TIRF
Status
Published
Research group
- Diabetes - Islet Patophysiology
ISBN/ISSN/Other
- ISSN: 0021-9533