Flow field-flow fractionation: Critical overview.

Summary, in English

This overview regards some critical issues in performing flow field-flow fractionation (flow FFF, FlFFF, AF4, HF-FlFFF, HF5). It includes the channel thickness, void time, channel-flow parabolic profile, channel-flow velocity gradient, uniformity of the cross-flow, sample injection time, relaxation/focusing time, width of sample starting zone, retention level, theoretical and experimental zone broadening, hydrodynamic threshold immobilisation/re-mobilisation, sample loss and adsorption, membrane fouling, sample mass overloading, problems with symmetrical channels, non-spherical sample particles, and method development. Good method development practice (GMDP) and good fractogram practice (GFP) is suggested.

• Author: Karl-Gustav Wahlund
• Department/s: Centre for Analysis and Synthesis
• Publishing year: 2013
• Language: English
• Pages: 97-112
• Publication/Series: Journal of Chromatography A
• Volume: 1287
• Links: http://dx.doi.org/10.1016/j.chroma.2013.02.028
• Document type: Journal article review
• Publisher: Elsevier
• Topic: Chemical Sciences
• Status: Published
• ISBN/ISSN/Other: ISSN: 0021-9673